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	<title>
	Comments on: DIY Digital Microfluidics for Automating Biology Protocols (sub-microliter droplets)	</title>
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	<link>http://88proof.com/synthetic_biology/blog/archives/280</link>
	<description>Genetically Engineered Organisms, Systems Biology, and Synthetic Biology from an Engineer&#039;s Viewpoint</description>
	<lastBuildDate>Wed, 09 Dec 2009 01:45:15 +0000</lastBuildDate>
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		<title>
		By: JonathanCline		</title>
		<link>http://88proof.com/synthetic_biology/blog/archives/280/comment-page-1#comment-81</link>

		<dc:creator><![CDATA[JonathanCline]]></dc:creator>
		<pubDate>Fri, 30 Oct 2009 03:03:28 +0000</pubDate>
		<guid isPermaLink="false">http://88proof.com/synthetic_biology/blog/?p=280#comment-81</guid>

					<description><![CDATA[In reply to &lt;a href=&quot;http://88proof.com/synthetic_biology/blog/archives/280/comment-page-1#comment-58&quot;&gt;Bruce&lt;/a&gt;.

1mm x 1mm pads with 0.5mm gap (or smaller).  Though, tried a bunch of different combinations there.  The pad size is related to droplet size ( 1 uL of water being well suited to 1.5mm pad size or so).   Gap width should be minimized as much as possible without causing shorts or sparks.  Sparking can be a big problem.]]></description>
			<content:encoded><![CDATA[<p>In reply to <a href="http://88proof.com/synthetic_biology/blog/archives/280/comment-page-1#comment-58">Bruce</a>.</p>
<p>1mm x 1mm pads with 0.5mm gap (or smaller).  Though, tried a bunch of different combinations there.  The pad size is related to droplet size ( 1 uL of water being well suited to 1.5mm pad size or so).   Gap width should be minimized as much as possible without causing shorts or sparks.  Sparking can be a big problem.</p>
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		<item>
		<title>
		By: Bruce		</title>
		<link>http://88proof.com/synthetic_biology/blog/archives/280/comment-page-1#comment-58</link>

		<dc:creator><![CDATA[Bruce]]></dc:creator>
		<pubDate>Thu, 30 Jul 2009 22:16:36 +0000</pubDate>
		<guid isPermaLink="false">http://88proof.com/synthetic_biology/blog/?p=280#comment-58</guid>

					<description><![CDATA[Jonathan, what are the pad and trace sizes you used as well as the gap between the pads?

Thanks, Bruce]]></description>
			<content:encoded><![CDATA[<p>Jonathan, what are the pad and trace sizes you used as well as the gap between the pads?</p>
<p>Thanks, Bruce</p>
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		<title>
		By: Low Cost Microcontroller-based Digital Microfluidics using &#8220;Processing&#8221; - 88 Proof Synth Bio Blog		</title>
		<link>http://88proof.com/synthetic_biology/blog/archives/280/comment-page-1#comment-57</link>

		<dc:creator><![CDATA[Low Cost Microcontroller-based Digital Microfluidics using &#8220;Processing&#8221; - 88 Proof Synth Bio Blog]]></dc:creator>
		<pubDate>Thu, 02 Jul 2009 03:49:08 +0000</pubDate>
		<guid isPermaLink="false">http://88proof.com/synthetic_biology/blog/?p=280#comment-57</guid>

					<description><![CDATA[[...] now tested the digital microfluidics board via microcontroller. The digital microfluidics board moves a liquid droplet via Electrowetting-on-Dielectric (EWOD).  The microcontroller switches the [...]]]></description>
			<content:encoded><![CDATA[<p>[&#8230;] now tested the digital microfluidics board via microcontroller. The digital microfluidics board moves a liquid droplet via Electrowetting-on-Dielectric (EWOD).  The microcontroller switches the [&#8230;]</p>
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			</item>
		<item>
		<title>
		By: JonathanCline		</title>
		<link>http://88proof.com/synthetic_biology/blog/archives/280/comment-page-1#comment-56</link>

		<dc:creator><![CDATA[JonathanCline]]></dc:creator>
		<pubDate>Tue, 23 Jun 2009 23:22:05 +0000</pubDate>
		<guid isPermaLink="false">http://88proof.com/synthetic_biology/blog/?p=280#comment-56</guid>

					<description><![CDATA[Lou -
In the bottom picture, the droplet moves right to left.
The droplet can be any water solution.  Also, water surrounded by oil also works in the research.

I will post another article with further details including circuit board layout files for easy fabrication.]]></description>
			<content:encoded><![CDATA[<p>Lou &#8211;<br />
In the bottom picture, the droplet moves right to left.<br />
The droplet can be any water solution.  Also, water surrounded by oil also works in the research.</p>
<p>I will post another article with further details including circuit board layout files for easy fabrication.</p>
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		<item>
		<title>
		By: Lou		</title>
		<link>http://88proof.com/synthetic_biology/blog/archives/280/comment-page-1#comment-55</link>

		<dc:creator><![CDATA[Lou]]></dc:creator>
		<pubDate>Tue, 23 Jun 2009 17:38:50 +0000</pubDate>
		<guid isPermaLink="false">http://88proof.com/synthetic_biology/blog/?p=280#comment-55</guid>

					<description><![CDATA[Interesting subject..
In the picture you have 6 vertical electrodes ( horizontal spark gap brakes ), is the droplet of the fluid (blue droplet on the right) going to travel in the horizontal direction
when sequencing individual electrodes?
What fluids have you tested so far?
surface tension, viscosity challenges?
Thank you for yours comments.
Lou]]></description>
			<content:encoded><![CDATA[<p>Interesting subject..<br />
In the picture you have 6 vertical electrodes ( horizontal spark gap brakes ), is the droplet of the fluid (blue droplet on the right) going to travel in the horizontal direction<br />
when sequencing individual electrodes?<br />
What fluids have you tested so far?<br />
surface tension, viscosity challenges?<br />
Thank you for yours comments.<br />
Lou</p>
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